News for dentistry professionals
25 Feb 2019
González M, Soler A, Gómez R, Blanc V, León R. The influence of Solobacterium moorei in a multispecies biofilm. DENTAID Research Center, Barcelona.
Species of the genera Fusobacterium, Porphyromonas, Treponema and Prevotella are frequently cited as responsible for the production of the main volatile sulphur compounds (VSCs) detected in halitosis.
However, a higher prevalence of Solobacterium moorei has recently been described in people with halitosis than in healthy individuals, suggesting that this species may be involved in bad breath.
In addition, S. moorei has the ability to generate VSCs in monospecies biofilms, although at lower concentrations than Fusobacterium nucleatum or Porphyromonas gingivalis.
The objective of this research was to study the effect of S. moorei on the production of volatile organic compounds (VOC) in multispecies biofilms.
Three different multispecies biofilms were developed under anaerobic conditions for six days in an artificial mouth system:
1. Type 1 Community (T1C) was made up of Streptococcus gordonii (Sg), Streptococcus oralis (So), Actinomyces naeslundii (An), Veillonella parvula (Vp), Aggregatibacter actinomycetemcomitans (Aa) and Fusobacterium nucleatum (Fn).
2. Type 2 Community (T2C) included Sg, So, An, Vp, Aa, Fn, Porphyromonas gingivalis (Pg) and Porphyromonas intermedia (Pi).
3. Type 3 Community (T3C) contained the same microbes as T2C plus S. moorei (Sm).
Agar, blood and Dentaid-1 plates were used to determine the bacterial load of each species. Mass spectrometry was used to analyse the gas produced by the biofilms.
The percentages of P. intermedia and P. gingivalis increased significantly with the participation of S. moorei in the biofilm (T2C versus T3C). Their presence significantly altered the production of VOCs and VSCs.
Dimethyl disulfide (DMDS) was the principal compound in the biofilms, and greatest production of this occurred in biofilms containing S. moorei.
DISCUSSION & CONCLUSION
The participation of S. moorei in a biofilm fosters the presence of pigmented species in the biofilm. This could be explained by the β-galactosidase activity of S. moorei, which would provide greater access to nutrients by proteolytic bacteria, such as P. gingivalis or P. intermedia.
Thus, S. moorei would facilitate integration of VSC-producing bacteria in the biofilm. On the other hand, it is necessary to study the odour power of the five VSCs that appeared in biofilms with S. Moorei to establish how closely associated they are to bad breath.
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